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Several recently determined non-peptidic ligand bound GLP-1R structures 13,14,16 have uncovered previously unknown binding pockets that may have implications in their pharmacological profiles such as biased signaling and allosteric agonism (Supplementary Fig

Calculations of pKa for individual Cys residues were performed with Propka 3 which gives an approximation of the pKa based on a solution of the Boltzmann equation (Rostkowski et al., 2011)

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